Summary
The study examined immune responses following vaccination with inactivated SARS CoV 2, revealing alterations in gene expression in immune cell types. These changes included heightened NF κB signalling and reduced type I interferon responses, resembling patterns seen in COVID 19 patients. Clinical laboratory findings post vaccination showed electrolyte imbalances, coagulation profile shifts, and renal dysfunction, suggesting a simulated infection. Monocytes exhibited increased inflammatory responses, indicating immune activation post vaccination. The Multi Variate Score (MVS) genes associated with infection were upregulated following vaccination, indicating a mimicry of infection. Positive MVS genes were expressed in monocytes, while negative genes were found in lymphocytes. Pathways linked to MVS gene sets were identified, underscoring the impact of vaccination on immune cell gene expression. Initial data suggested enhanced type I interferon responses in peripheral blood mononuclear cells (PBMCs) post vaccination. Antibiotics may affect vaccine effectiveness. Although different cohorts displayed varying antibody profiles, PBMC results were similar. Detailed immune analyses remain crucial for vaccine development and understanding long term health implications. The study stresses the importance of monitoring individuals with underlying health conditions during vaccination.